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By the time Sanger sequencing was commercialized, the groundwork for an entirely new sequencing chemistry was already well underway. In 1985, Swedish biochemists Pål Nyren and Arne Lundin published a paper illustrating a procedure that measured the concentration of a molecule, called pyrophosphate (PPi), using an enzymatic cascade that emits light. In early 1986, Nyren realized that the method he’d helped develop could be applied to DNA sequencing, because PPi is naturally produced as a byproduct of DNA synthesis.5。业内人士推荐下载安装 谷歌浏览器 开启极速安全的 上网之旅。作为进阶阅读
第二百九十一条 根据海上保险合同向保险人请求保险赔偿的请求权，时效期间为二年，自知道或者应当知道海上保险事故发生之日起计算。